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HRP標記鏈霉親和素

Concentration:         1 mg/ml, Buffer=0.01M TBS(pH 7.4) with 1% BSA,0.03% Proclin300 and 50% Glycerol.

Application Notes:    ELISA=1:200-2000  IHC=1:200-1000

ICC=1:200-1000   WB=1:200-500

optimal dilutions/concentrations should be determined by the end user.

Storage Instructions:  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 °C.

Background:

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

This enzyme conjugate solution can be used to stain biotinylated antibodies.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

HRP標記鏈霉親和素

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